Cells, Free Full-Text

Identifying tissue-specific molecular signatures of active regulatory elements is critical to understanding gene regulatory mechanisms. In this study, transcription start sites (TSS) and enhancers were identified using Cap analysis of gene expression (CAGE) across endometrial stromal cell (ESC) samples obtained from women with (n = 4) and without endometriosis (n = 4). ESC TSSs and enhancers were compared to those reported in other tissue and cell types in FANTOM5 and were integrated with RNA-seq and ATAC-seq data from the same samples for regulatory activity and network analyses. CAGE tag count differences between women with and without endometriosis were statistically tested and tags within close proximity to genetic variants associated with endometriosis risk were identified. Over 90% of tag clusters mapping to promoters were observed in cells and tissues in FANTOM5. However, some potential cell-type-specific promoters and enhancers were also observed. Regions of open chromatin identified using ATAC-seq provided further evidence of the active transcriptional regions identified by CAGE. Despite the small sample number, there was evidence of differences associated with endometriosis at 210 consensus clusters, including IGFBP5, CALD1 and OXTR. ESC TSSs were also located within loci associated with endometriosis risk from genome-wide association studies. This study provides novel evidence of transcriptional differences in endometrial stromal cells associated with endometriosis and provides a valuable cell-type specific resource of active TSSs and enhancers in endometrial stromal cells.

Lactobacillus delbrueckii ssp. bulgaricus B-30892 can inhibit cytotoxic effects and adhesion of pathogenic Clostridium difficile to Caco-2 cells, Gut Pathogens

Streptomyces cell-free systems for natural product discovery and engineering - ScienceDirect

Cancers, Free Full-Text

Cells, Free Full-Text

Cells, Free Full-Text

The cell-free system: A new apparatus for affordable, sensitive, and portable healthcare - ScienceDirect

Cancers, Free Full-Text

An illustration of the full-duplex cell-free massive MIMO system.

Advancing synthetic biology through cell-free protein synthesis - ScienceDirect

PDF) Comparative separation methods and biological characteristics of human placental and umbilical cord mesenchymal stem cells in serum-free culture conditions

Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles

Full-spectrum cell-free RAN for 6G systems: s

Cells, Free Full-Text

Cell-Free Protein Expression

Labile coat: reason for noninfectious cell-free varicella-zoster virus in culture. - Abstract - Europe PMC